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. 2011 Mar;162(5):1136–1142. doi: 10.1111/j.1476-5381.2010.01110.x

Figure 2.

Figure 2

Perfusion of hearts with N,N,N′,N′-tetramethylphenylene-1,4-diamine (TMPD) has no effect on ischaemia-induced release of cytochrome c from mitochondria into cytosol. (A) Cytochrome c concentration in mitochondria; (B) cytochrome c concentration in cytosolic fractions from control and ischaemic hearts. Hearts were pre-perfused for 15 min with 50 µM TMPD prior to induction of stop-flow ischaemia. The cytochrome c concentration in isolated mitochondria was measured spectrophotometrically as described in Methods. Cytosolic concentrations of cytochrome c were measured using elisa. *Statistically significant effect if compared with control (P < 0.01). Means ± SE of 4–9 separate experiments are presented.