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. 2011 Mar;162(5):1136–1142. doi: 10.1111/j.1476-5381.2010.01110.x

Figure 4.

Figure 4

Perfusion of the hearts with N,N,N′,N′-tetramethylphenylene-1,4-diamine (TMPD) prevents ischaemia-induced apoptosis. Hearts were pre-perfused for 15 min with 50 µM TMPD prior to induction of 60 min stop-flow ischaemia. After ischaemia, hearts were fixed and stained using the Cardiotask kit for determination of cardiomyocytes with fragmented DNA. Number of TUNEL-positive cells is expressed as the percentage of total number of cardiomyocytes in tissue sections investigated. *Statistically significant effect (P < 0.01) if compared with control. #Statistically significant effect of TMPD (P < 0.01) if compared with ischaemia alone. Means ± SE of data from three hearts in each condition are presented. TUNEL, dUTP nick end labelling.