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Perfusion of the hearts with N,N,N′,N′-tetramethylphenylene-1,4-diamine (TMPD) prevents ischaemia-induced necrosis. Coronary effluents during reperfusion were collected for the determination of creatine kinase activity as described in Methods. *Statistically significant effect (P < 0.01) if compared with control. #Statistically significant effect of TMPD (P < 0.01) if compared with ischaemia/reperfusion in the absence of TMPD. Means ± SE of 5–9 separate experiments are presented.
