Effect of melatonin (10–1000 nM) on the maximal outflow of 5-HT and 5-HIAA from the muscle layer-free mucosal preparations evoked by 1 µM βAla-NKA-(4-10). Height of columns: βAla-NKA-(4-10)-evoked maximal 5-HT and 5-HIAA outflow, expressed as per cent of the mean outflow of first two collections (100–120 min). Results are the means ± SEM from 6 to 10 experiments. One-way analysis of variance followed by Newman–Keuls post hoc test, *P < 0.05, **P < 0.01, significantly different from the control. 5-HT, 5-hydroxytryptamine; 5-HIAA, 5-hydroxyindoleacetic acid; βAla-NKA-(4-10), [β-Ala8]-neurokinin A4-10.