Figure 7.

Effect of glucocorticoid-induced tumour necrosis factor receptor family-related protein (GITR) inhibition on inducible nitric oxide synthase (iNOS) expression, nitrosative stress and poly ADP-ribose polymerase activation. Twenty-four hours after cerulein injection, immunohistochemical localization of iNOS (A–D) nitrotyrosine (E–H) and poly ADP-ribose (PAR) (I–N) in the pancreatic tissue of GITR^+/+ mice showed a positive iNOS (B) nitrotyrosine (F) and PAR (L) staining, when compared with sham operated mice (A, E and I respectively). After cerulein administration, in the pancreatic tissue of GITR^−/− mice and Fc-GITR co-treated (6.25 µg·mouse⁻¹, by mini-osmotic pump) GITR^+/+ mice, there was no detectable immunostaining for iNOS (C and D, respectively), nitrotyrosine (G and H, respectively) and PAR (M and N respectively). One representative experiment out of three is shown.