Figure 8. The failure of the Isc system during H2O2 stress is not due to a lack of Isc synthesis.
(A) Expression after aeration of a transcriptional lacZ fusion placed behind the sufNI promoter that lacks the OxyR binding site. Induction by apo-IscR is greatest in a suf mutant. (B) Expression after aeration of a transcriptional lacZ fusion placed behind the iscRSUA promoter. (C) IscU polypeptide was detected by western blot analysis using an anti-IscU antibody during aerobic growth. The experiments were performed using glucose/casamino acids medium.