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. 2011 Mar 7;192(5):781–795. doi: 10.1083/jcb.201009043

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© 2011 Yabuta et al.

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Figure 3. — Tdrd5 knockout leads to male sterility. (A) The gene-targeting strategy for Tdrd5. The correct targeting generates ∼7.0-kb bands when digested with EcoRV and probed with the 5′ probe (green boxes). (B) Southern blot for the detection of the gene targeting. (C and D) Northern (C) or Western (D) blots of Tdrd5^+/+ and Tdrd5^−/− testes for Tdrd5 or TDRD5 expression. (C) A, probe A (5′); B, probe B (3′) as shown in Fig. 1 A. T5-1, -2, and -3, Tdrd5 isoforms 1, 2, and 3. (E) Testes of Tdrd5^+/+ and Tdrd5^−/− mice of 4- and 8-wk of age. (F) The weight (the averages with SD; error bars) of Tdrd5^+/+ and Tdrd5^−/− testes (8 wk old). (G) Histology of Tdrd5^+/+ and Tdrd5^−/− testes (top) and epididymis (bottom) stained by hematoxylin and eosin. (H) Alkaline phosphatase staining of Tdrd5^+/+ and Tdrd5^−/− male (left) and female (right) gonads at E12.5. Bars: (E) 2 mm; (G) 50 µm; (H) 300 µm.