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. 2011 Mar 7;192(5):781–795. doi: 10.1083/jcb.201009043

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© 2011 Yabuta et al.

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Figure 7. — Abnormal activation of LINE-1 in Tdrd5^−/− germ cells. (A) Northern blot of retrotransposon (LINE-1, IAP, SINE-B1) expression in Tdrd5^+/+ and Tdrd5^−/− testes at P14. (B) Q-PCR analysis of retrotransposon expression in Tdrd5^+/+ and Tdrd5^−/− testes at P14 shown by the log₂ scale (the average with SD; error bars). The expression levels in Tdrd5^+/+ animals are set as zero. (C) Immunofluorescence analysis of ORF1p in prospermatogonia (E17.5) and zygotene and pachytene spermatocytes in Tdrd5^+/+ and Tdrd5^−/− mice. Arrowheads indicate ORF1p-strong-positive spots. Bars, 5 µm. (D) Immunofluorescence analysis of ORF1p at a severely damaged locus of a Tdrd5^−/− testis. Bar, 10 µm. (E) Methylation-sensitive Southern blot analysis of DNA methylation of LINE-1 (at P12 and P25) and IAP (at P25) promoters, and minor and major satellite sequences (at P25) in Tdrd5^+/+ and Tdrd5^−/− mice.