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. 2011 Mar 7;192(5):797–811. doi: 10.1083/jcb.201007014

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© 2011 Laursen et al.

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Figure 9. — hnRNP-K is found in an RNA-dependent complex with hnRNP-A2. (A) Immunoprecipitation of hnRNP-K from lysates of oligodendrocyte precursors (DAY 0), and mature oligodendrocytes (DAY 4), detected with antibodies against hnRNP-K and A2 as indicated. At each time point, the experiments were performed on PDL or laminin substrates, with the hnRNP-K immunoprecipitation lane labeled K and the control input and IgG lanes also shown. Note that in both precursors and mature cells grown on either substrate hnRNP-A2 is coimmunoprecipitated with hnRNP-K. (B) Oligodendrocytes grown in culture for 4 d on PDL or laminin substrates and immunolabeled with antibodies against hnRNP-K, hnRNP-A2, and MBP as shown. Note that the two hnRNPs colocalize in granule-like structures within the MBP+ myelin sheets. (C) Immunoprecipitation of hnRNP-K from lysates of mature oligodendrocytes with and without prior RNase treatment followed by Western blotting for hnRNP-K and A2, as indicated. Note that RNase treatment abolished coimmunoprecipitation of hnRNP-A2 with hnRNP-K, as shown in the two right-hand lanes. Input and IgG controls are shown in the three left-hand lanes.

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