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. 2011 Mar 7;192(5):855–871. doi: 10.1083/jcb.201007118

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© 2011 Bolhy et al.

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Figure 4. — hNup133 contributes to dynactin anchoring at the NE at the G2/M transition via CENP-F and NudE/EL. (A) GFP-hNup133_CTD or GFP_3x-mNup133 cells (a) or wild-type HeLa cells (b) transfected with the indicated siRNA duplexes were processed for immunofluorescence using anti-p150^Glued and anti–phospho-H3 antibodies. Bars, 10 µm. See also Fig. S3. (B) Schematic representation of the interaction networks connecting Nup133 to dynein/dynactin. Proteins are represented on approximate scale except for CENP-F. Boxes indicate the minimal domains involved in the interactions between Nup133 and CENP-F (black boxes; this paper and Zuccolo et al., 2007), CENP-F and NudE/EL (gray boxes), and between NudE/EL and dynein (dashed area overlapping with the CENP-F interaction domain; Liang et al., 2007; Stehman et al., 2007; Vergnolle and Taylor, 2007). Although not represented on this scheme, association of CENP-F with the pool of Nup133 localized on the nuclear side of NPCs cannot be excluded.