Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Mar 7;192(5):855–871. doi: 10.1083/jcb.201007118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Bolhy et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 9. — Centrosome disconnection from the NE causes transiently aberrant spindle structures. (A) Cells stably expressing GFP-hNup133_CTD and GFP-CENP-A (green) were transfected with a plasmid encoding the microtubule-binding protein mCherry-MAP4 (red) and with scramble (a) or hNup133 (b and c) siRNAs. Cells were imaged from prophase on. Top panels: mCherry-MAP4 signal; bottom panels: overlay of the mCherry-MAP4 and GFP-CENP-A + GFP-hNup133_CTD signals (note that the GFP-hNup133_CTD signal is hardly detectable over the GFP-CENP-A signal). Time is in min:sec. Times of NEBD, metaphase plate formation, and metaphase/anaphase transition are indicated. Bar, 10 µm. See also Videos 7–9. (B) Time spent from chromosome condensation to metaphase/anaphase transition for cells stably expressing GFP-hNup133_CTD and GFP-CENP-A treated with scramble or hNup133 siRNA duplexes as indicated. Each dot represents a single cell, and the dashed line represents the average mitotic duration. Cells that display major mitotic defects (red dots) or minor chromosome segregation defects (one mis-segregated or lagging chromosome; black dots) are indicated. The number of cell quantified, the average mitotic duration, and standard deviation are indicated. ns, the difference between control and Nup133-depleted cells was not statistically significant as determined using the Student’s t test. (C) Analysis of centrosome localization (arrows) in prometaphase and metaphase U2OS cells treated with scramble or NudE/EL siRNA duplexes. Cells were fixed and then stained with anti-pericentrin (red), anti–phospho-H3 (green), and RanGAP1 (gray) antibodies. RanGAP1 that localizes at NPCs in interphase and on the mitotic spindle in mitosis was used to simultaneously assess NPC disassembly and spindle formation. Representative cells at various stages of prometaphase and metaphase are presented. The percentage of mitotic cells displaying the indicated phenotypes is indicated. Bar, 10 µm.