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. 2011 Mar 7;192(5):873–882. doi: 10.1083/jcb.201009132

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© 2011 Choi et al.

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Figure 7. — NADH and dopamine accumulation underlie hypersensitivity of Ndufs4^−/− neurons to rotenone. Mesencephalic neurons were cultured from individual E14 Ndufs4^+/+ and Ndufs4^−/− embryos. (A) Deletion of the Ndufs4 gene increases basal intracellular NADH content. (B) 8-h 10-nM rotenone–induced intracellular dopamine accumulation is significantly higher in Ndufs4^−/− cultures than in Ndufs4^+/+ cultures. (C) 10 nM taxol inhibits 8-h 10-nM rotenone–induced dopamine accumulation in both Ndufs4^+/+ and Ndufs4^−/− cultures. (D) Pretreatment with 0.2-mM α-methyl-ρ-tyrosine (AMPT) protects dopamine neurons cultured from both Ndufs4^+/+ and Ndufs4^−/− embryos against 5-nM rotenone toxicity for 24 h. Data are means ± SEM. n = 3–4; *, P < 0.05; **, P < 0.01.