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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 27;78(4):1038–1054. doi: 10.1111/j.1365-2958.2010.07389.x

Fig. 3.

Fig. 3

Effects of extracellular iron and acuM deletion on sreA and hapX mRNA levels. (A) A. fumigatus Af293 was grown in Aspergillus minimal medium (AMM) containing ferrozine and FeSO4 (+Fe) or AMM containing ferrozine without FeSO4 (−Fe) for 24 h, after which the relative transcript levels of the indicated genes were determined by real-time PCR. (B and C) The indicated strains of A. fumigatus were grown for 24 h in AMM containing ferrozine without FeSO4, and then the relative transcript levels of sreA (B) and hapX (C) were determined by real-time PCR. Results are the mean ± SD of three biological replicates, each tested in triplicate. *P < 0.05 compared to organisms grown in the presence of FeSO4; †p < 0.05 compared to Af293 or the ΔacuM∷acuM complemented strain.