Fig. 5.
Deletion of acuM resulted in decreased growth in a low iron medium, impaired iron incorporation, and reduced extracellular siderophore production. (A) Dry weight of the indicated strains after 40 h of incubation in Sabouraud broth containing phenanthroline with (+Fe) or without FeSO4 (−Fe). (B) Relative incorporation of 55FeCl3 after 1 h by washed germlings of the indicated strains. (C) Extracellular siderophore activity of the indicated strains after 30 h of growth. Results are mean ± SD of three (A and B) or four (C) experiments, each performed in triplicate. *P < 0.001 compared to Af293 and the ΔacuM∷acuM complemented strain.