Table 1.
List of A. fumigatus genes that are predicted to specify transcription factors, and for which null mutants were constructed.
| Strain | A. fumigatus Gene | Ortholog | Function of ortholog | Virulence of null mutant (Median survival time in days)a |
|---|---|---|---|---|
| Af293 (wild-type) | 8.5 | |||
| ΔAfu1g02860 | Afu1g02860 | Fusarium graminearum TRI6 | Regulator of mycotoxin production (Pinson-Gadais et al., 2008; Proctor et al., 1995; Seong et al., 2009) | 7.5 |
| ΔAfu1g04140 | Afu1g04140 | A. fumigatus specific transcription factor | 7.5 | |
| ΔAfu2g04600 | Afu2g04600 | Aspergillus. parasticus SugR | Regulator of sugar utilization (Yu et al., 2000) | 7.5 |
| ΔacuM | Afu2g12330 (acuM) | Saccharomyces cerevisiae Rds2; Candida albicans Cwt1; Aspergillus nidulans AcuM | Regulator of cell wall integrity and gluconeogenesis (Moreno et al., 2003; Moreno et al., 2007a; Moreno et al., 2008; Soontorngun et al., 2007) | 12.0b |
| ΔacuM ΔsreA | Afu2g12330 (acuM) Afu5g11260 (sreA) | A. nidulans SreA | Negative regulator of iron homeostasis | NDc |
| ΔAfu2g16310 | Afu2g16310 | Fungal specific transcription factor | 8.0 | |
| ΔAfu4g09710 | Afu4g09710 | A. nidulans NosA | Regulator of sexual development (Vienken and Fischer, 2006) | 8.0 |
| ΔsteA | Afu5g06190 (steA) | S. cerevisiae Ste12; C. albicans Cph1 | Regulator of filamentation and mating (Braun and Johnson, 2000; Lewis et al., 2002; Liu et al., 1994; Ramer and Davis, 1993; Vallim et al., 2000) | 9.0 |
| ΔAfu5g10130 | Afu5g10130 | A. fumigatus specific transcription factor | 7.0 | |
| ΔAfu6g07170 | Afu6g07170 | A. nidulans RosA | Regulator of sexual development, repressor of NosA (Vienken et al., 2005) | 10.0 |
| Δyap1 | Afu6g09930 (yap1) | S. cerevisiae Yap1 | Regulator of oxidative stress tolerance (Delaunay et al., 2000) | 9.0 |
| ΔAfu8g05460 | Afu8g05460 | Up-regulated in heat shock (Do et al., 2009) | 7.0 | |
| Afu2g10770 | Magnaporthe. grisea Con7 | Essential for appressorium formation and disease-related morphogenesis (Odenbach et al., 2007) | ---d |
a
Survival of G. mellonella larvae after infection with 106 conidia of the indicated null mutant Data are the combined results two experiments, each using 20–30 larvae per strain
b
P < 0.001 compared to larvae infected with Af293
c
Not determined
d
Deletion mutant for this gene was unable to be constructed