Table 3.
List of genes involved in siderophore synthesis and transport, and iron uptake that were down-regulated in the ΔacuM mutant compared to Af293.
| A. fumigatus gene | Fold-change in mRNA level in the ΔacuM mutant | Putative function | ||
|---|---|---|---|---|
| Microarray (18 h)a | Microarray (24 h)a | Real-time PCR (24 h)b | ||
| Afu1g17270 (fre2) | −3.2 | −2.4 | −2.3 | Ferric-chelate reductase |
| Afu5g03800 (ftrA) | −2.8 | −1.6 | −1.6 | High-affinity iron permease |
| Afu2g07680 (sidA) | −4.0 | −1.4 | −4.2 | L-ornithine N5-oxygenase |
| Afu1g17200 (sidC) | −1.7 | −1.2 | −1.7 | Nonribosomal peptide synthase |
| Afu3g03420 (sidD) | −3.3 | −2.0 | −3.1 | Fusarinine C nonribosomal peptide synthetase |
| Afu3g03650 (sidG) | −3.0 | −1.9 | −11.4 | Fusarinine C acetyltransferase |
| Afu3g03400 (sidF) | −3.4 | −2.1 | −7.2 | Siderophore biosynthesis acetylase |
| Afu3g03390 | −2.3 | −1.7 | −5.0 | Siderophore biosynthesis lipase/esterase |
| Afu3g03640 (mirB) | −4.0 | −2.7 | −4.1 | Siderophore transporter |
| Afu7g06060 (sit1) | −2.2 | −2.1 | −2.1 | Siderophore transporter |
| Afu7g04730 | −2.4 | −1.8 | −2.1 | Siderophore transporter |
| Afu6g07720 (acuF) | −9.4 | −6.4 | −16.2 | Phosphoenolpyruvate carboxykinase |
| Afu4g11310 (fbp1) | −2.9 | −1.7 | −7.2 | Fructose-1,6-bisphosphatase |
a
Mean of 4 biological replicates in RPMI 1640 medium
b
Mean of 3 biological replicates in AMM without iron and with 300 µM ferrozine