Skip to main content
. Author manuscript; available in PMC: 2012 Jan 5.
Published in final edited form as: Virology. 2010 Oct 28;409(1):113–120. doi: 10.1016/j.virol.2010.09.018

Figure 4. Human Trim5α affecting TAB2 levels is dependent on its RING and B-box domains, but not on its PRY-SPRY domain.

Figure 4

(A) Diagram showing domains of Trim5α, Trim5γ, Trim22, and the chimeras constructed between Trim5 and Trim22. Trim22R-5α indicates Trim22 RING in a Trim5α background. Trim5R-22 indicates Trim5 RING in a Trim22 background. Trim5RB-22 indicates a Trim5 RING and B-box in a Trim22 background. (B) 293T cells were co-transfected with constant amounts of human TAB2 (2ug DNA per lane) and with increasing amounts of either empty vector LPCX or with human Trim5γ (gradient of 0.06ug, 0.2ug, 0.6ug DNA per lane). The doublet band of Trim5γ has been reported before (Stremlau et al., 2004). (C) 293T cells were co-transfected with constant amounts of human TAB2 and with increasing amounts of either human Trim5α, or human Trim22, or the chimeras Trim22R-5α, Trim5R-22, or Trim5RB-22. DNA transfected same as Fig4B. Diagrams of each Trim construct is shown below their respective immunoblot for conveniance. All constructs contain HA tags. Lysates were probed with anti-HA antibodies, then stripped and re-probed with anti-actin.