Figure 2.

Hierarchical clustering of top isolates and Trichoderma reesei. Heatmap showing mean activities and clustering of the top isolates on each substrate and T. reesei when assayed for hydrolysis of 14 polysaccharide substrates [SG, switchgrass; CS, corn stalk; EGG/SG, eastern gammagrass/switchgrass mix; BBS/SG, big bluestem/SG mix; TF, tall fescue; AXW, arabinoxylan (wheat); AXO, arabinoxylan (oat), XY, xylan (birch); RC, reed canary grass; BMCC, bacterial microcrystalline cellulose; FP, filter paper; PTSGB, base pretreated SG; PTSGA, acid pretreated SG; PCS, acid pretreated corn stover]. Each substrate was hydrolyzed for two lengths of time and the mean of those two timepoints was used for clustering. Selected isolates showed the highest activity on at least one substrate and at least one timepoint. Measured reducing sugars were standardized within substrates by subtracting the substrate mean and dividing by the standard deviation. Dendrogram and ordering was determined using the distance matrix computation (dist) and hierarchical clustering (hclust) functions in R. Red colors indicate values greater than the substrate mean, while blue colors indicate values less than the mean. Column Z-score and color intensity indicate how many standard deviations the isolate mean is from the substrate mean.