Table 4.
Effect of anti-co-receptors antibodies on Treg cells, IL-10 and IFN-γ production
| R (n = 12) | AR (n = 10) | A (n = 11) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Derp1 | Derp1+ anti-ICOS | Derp1+ anti-CTLA4 | Derp1+ anti-CD28 | Derp1 | Derp1+ anti-ICOS | Derp1+ anti-CTLA4 | Derp1+ anti-CD28 | Derp1 | Derp1+ anti-ICOS | Derp1+ anti-CTLA4 | Derp1+ anti-CD28 | |
| CD3+IL-10+(%) | 3.82 ± 0.49 | 3.79 ± 0.30 | 4.66 ± 1.05 | 3.31 ± 0.32 | 3.80 ± 0.41 | 3.54 ± 0.46 | 3.96 ± 0.50 | 3.60 ± 0.52 | 5.03 ± 0.68 | 4.42 ± 0.56 | 4.42 ± 0.64 | 4.37 ± 0.64 |
| CD4+CD25Hi+ Foxp3+ (%) | 4.25 ± 0.45 | 4.49 ± 0.65 | 4.24 ± 0.57 | 3.57 ± 0.65 | 3.61 ± 0.70 | 4.21 ± 1.13 | 3.87 ± 0.83 | 2.78 ± 0.83 | 2.65 ± 0.47 | 2.47 ± 0.38 | 2.55 ± 0.40 | 1.75 ± 0.29 |
| CD3+IFN-γ+ (%) | 7.48 ± 1.15 | 6.13 ± 0.75 | 7.24 ± 1.11 | 7.63 ± 0.98 | 12.26 ± 1.35 | 11.86 ± 1.01 | 13.99 ± 1.84 | 13.64 ± 1.36 | 11.90 ± 1.89 | 10.43 ± 1.78 | 12.35 ± 1.74 | 12.32 ± 1.98 |
| CD3+IL-4+ (%) | 4.22 ± 0.55 | 2.01 ± 0.26* | 3.67 ± 0.94 | 2.33 ± 0.40* | 3.29 ± 0.49 | 2.21 ± 0.15*** | 2.85 ± 0.34 | 2.41 ± 0.22** | 3.53 ± 0.50 | 2.56 ± 0.54 | 2.92 ± 0.51 | 3.11 ± 0.95 |
PBMC from allergic non asthmatics (R, n = 12), allergic asthmatics (AR, n = 10) and non allergic asthmatics (A, n = 11), were stimulated with Derp 1 and cultured in the presence or absence of anti-ICOS, anti-CTLA4 or anti-CD28 antibodies. Treg cells, IL-10 and IFN-γ production by T-cells were assessed by flow cytometry. Results are expressed as mean of total T-cells ± SE and compared versus absence of anti-co-receptors antibodies conditions. R = allergic rhinitis; A R = allergic asthma and rhinitis. * = p < 0.05, ** = p < 0,01, *** = p < 0,001.