Figure 3.

In more than 90% of the experiments desensitization was lost immediately after patch excision. The series of traces obtained in the same patch with the same pressure stimulus. Buffers: KCl (pipette)/NaCl (bath) Command voltage 40 mV. Pipette BN 5. (A) Cell-attached configuration (B) membrane vesicle blocks the tip (C) inside-out patch, immediately upon air exposure (D) inside-out patch, 5 minutes upon excision. The gradual current increase in (C) is likely due to flow of the membrane patch into the pipette, so more area and more channels were available. (D) Shows stabilization of the new patch position.