Figure 5.

The responses of TREK-1 Δ76–125/Δ334–411 deletion mutant lacking both the extracellular loop and last 77 residues from the C-terminus. (A) Activation by pressure gradient. (B) Spontaneous activity at +40 mV. (C) Spontaneous activity in response to a ±100 mV voltage ramp. Deletion of the C-terminus removes voltage dependency. (D) Time-dependent effect of arachidonic acid (AA , 20 µm) added in the pipette, the upper trace was recorded immediately after seal formation and the bottom trace was recorded 20 min after. Without AA, the pressure stimulus invoked only a small current. Color versions of our figures can be seen at http://www.landesbioscience.com/journals/channels/ar ticle/13906/