Figure 6.

Elucidating the role of cysteines in TREK-1. (A) Mercaptoethanol (β-ME) present in the pipette shows no significant effect on activation of WT TREK-1 by pressure. (B) The C159S mutation introduced on the WT or Δ76–125/Δ334–411 background makes channel inactive under standard pressure protocol, but the mutants show small residual activity when the patch is pre-treated with AA. (C) C219A/Δ76–125/Δ334–411 mutant showed substantial spontaneous activity and was activated essentially as WT under standard pressure protocol. Color versions of our figures can be seen at http://www.landesbioscience.com/journals/channels/article/13906/