Figure 4.
Isolated NK cells, DC and CTL migrate toward virus filtered reoTCM. 5 × 105 NK cells (A), DC (B) or CTL (C) were resuspended in RPMI + 0.5% human AB serum + 1% L-Glutamine and placed in a 5 μm (NK + CTL) or 8 μm (DC) Thincerts™ to separate cells from virus filtered reoTCM/non-reoTCM. Migration was assessed after 3 hours by labelling cells with CD11c-PE (DC), CD56-PE/CD3-FITC (NK cells) or CD3-FITC/CD8-PerCP (CTL) and then using Trucount™ tubes to provide an internal counting control. A cell:bead ratio was determined for each tube and a migration index calculated by normalising this ratio to those of non-reoTCM controls. Data represents means of triplicate wells +/- SEM and is representative of 4 independent donors. * indicates P < 0.05 by Student's t-test.