Figure 4.

Kinetics of repopulating T cells in peripheral blood: (A) and (B) show the kinetics of absolute numbers of repopulating CD8⁺ and CD4⁺ T cells, respectively, in the peripheral blood of patient 1 (○) and patient 2 (●) and Sim/RATG patients (living donor-kidney transplant recipients given the same induction therapy but not MSCs, as controls, ■) from baseline (pretransplant) to day 360 posttransplant. Data are means ± SEM. *P < 0.05 versus pretransplant. Percentages of memory CD45RO⁺RA⁻ T cells within CD8⁺ T cells (C) and of regulatory CD25^highFoxP3⁺CD127⁻ cells within CD4⁺ T cells [Treg, (D)] and the ratio of Treg/memory CD8⁺ T cells (E) from patient 1 (○) and patient 2 (●) and from Sim/RATG patients (■) from baseline (pretransplant) to day 360 posttransplant. Data are means ± SEM. *P < 0.05 versus pretransplant. Expression of FoxP3 and CD127 antigens (dot plots on the right) by gated CD4⁺ and CD25^high T cells (dot plots on the left) at day 360 posttransplant from patient 1 (F) and from a living donor-kidney transplant recipient given the same induction therapy but not MSCs (G) is shown. Numbers in outlined areas indicate percentage of FoxP3⁺CD127⁻ T cells.