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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Dec 16;405(3):338–343. doi: 10.1016/j.bbrc.2010.12.036

Figure 2.

Figure 2

Panel A. Prekallikrein activation on endothelial cells. Pulmonary artery endothelial cells (HPAEC, 3×104 cells/well) were platted and cultured in 96 well plates. Cells were incubated with increasing concentrations of HK and PK. Panel B. Effect of anti-PRCP antibody on PK activation on HPAEC. Triplicate samples of cells were incubated with increasing concentrations of anti-PRCP antibody in the absence or presence of the complex of HK and PK. The control was without anti-PRCP antibody. Panel C. Schematic illustration of the pathways involved in the conversion of bradykinin to its respective metabolites. Panel D. The generation of BK 1–9, BK 1–8, and BK 1–7 were determined on HPAEC by LC/MS. The negative control was HK, a BK precursor.