FIG. 2.

Comparison of screening performances between absorbance and fluorescence methods. (A) Detailed protocol for fluorescence quenching method in 384-well plates. (B) Representative data from the side-by-side pilot screen for absorbance method and fluorescence method. Each dotted line represents three times standard deviation from the negative control. Data points for known inhibitors (as determined from previous 96-well assays) are circled. Fluorescence based method successfully identified all of the five known inhibitors whereas absorbance based method only detected one. The fluorescence method yielded ~4-fold higher signal-to-noise ratio than the absorbance method.