Fig. 3.

Reactivation of risperidone-inactivated h5-HT₇ receptors. Four inactivating antagonists and six non-inactivating antagonists were tested for the ability to reverse risperidone inactivation of the h5-HT₇ receptor. Cells were exposed to 10 nM risperidone, thoroughly washed, then exposed to 1 μM of drug for 90 min, thoroughly washed again, and stimulated with 10 μM 5-HT (black bars; see “Methods” section). The four inactivators (risperidone, 9-OH-risperidone, methiothepin, and bromocriptine) as well as ICI169369 did not reactivate the receptor; all other drugs reactivated the receptor as defined by sensitivity to 10 μM 5-HT. The results are the mean±SEM of three experiments performed in triplicate (*p<0.001 vs. control, one-way ANOVA with Dunnett’s post-test)