Fig. 9.

Wash-resistant inhibition of h5-HT₇ radioligand binding after exposure of intact cells to drugs followed by homogenization. h5-HT₇ receptor-expressing cells were exposed to either 1 μM drug or buffer (control) for 30 min. Cells were washed, homogenized, and assayed for radioligand binding using 2 nM [³H]5-HT. Risperidone and 9-OH-risperidone inhibit 50% of the binding while methiothepin and bromocriptine inhibit all of the binding; exposure to clozapine and mesulergine did not affect binding. Results are the means±SEM of three independent experiments performed in triplicate.*p<0.001, determined by one-way ANOVA with Dunnett’s post-test