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. 2011 Mar 9;6(3):e17392. doi: 10.1371/journal.pone.0017392

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Toth et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(4a) Representative merged image of PRE at 40× magnification with inset box showing (a-i) DAPI⁺ nuclei, (a-ii) Pax7⁺ nuclei, (a-iii) no p-STAT3 stained nuclei and (c-iiii) a merged image. (4b) Representatvie merged image of T3 at 40× magnification with inset box showing (b-i) DAPI⁺ nuclei, (b-ii) Pax7⁺ nuclei, (b-iii) p-STAT3⁺ nuclei and (b-iiii) a merged image showing co-localiztion. Note that punctate p-STAT3 is not present at PRE but co-localizes with Pax7 at T3 and that there is an increase in diffuse fiber staining that occurs from PRE to T3. (4c) Percentage of p-STAT3⁺ SC as quantified over the time course peaking at T24. Values are reported as mean ± S.E.M. *p<0.05 vs. PRE.