Table 1. Primers used in the study.
| Primer Name | Sequence Details (5′→3′) ** |
| PknBc T171/173D FP | CGGCAACAGCGTGGACCAGGACGCAGCAGTGATCG |
| PknBc T171/173D RP | CGATCACTGCTGCGTCCTGGTCCACGCTGTTGCCG |
| PknA FP | TGATCGAAGCCGGAATTCAGGGGGAACCATGA EcoR1 |
| PknAc RP | AGCACCCCCGCGGCCGCGAGCAGCGCTCACTGACCGGAC Not1 |
| PstPc D38G FP | CTATTGGCCCTGGCCGGCGGCATGGGTGGGCAT |
| PstPc D38G RP | ATGCCCACCCATGCCGCCGGCCAGGGCCAATAG |
| PstPc R20G FP | GATCGCGGCTTGGTAGGCGCCAACAACGAAGACTCGGTC |
| PstPc R20G RP | GACCGAGTCTTCGTTGTTGGCGCCTACCAAGCCGCGATC |
| PstPc D229G FP | GGCGGCGGCCCCGGCAACGTCACTGTCGTCGTC |
| PstPc D229G RP | GACGACGACAGTGACGTTGCCGGGGCCGCCGCC |
| PstPc T5A FP | GGAGAGTGGCGCGCGTGGCCCTGGTCCTGCGATAC |
| PstPc T5A RP | GTATCGCAGGACCAGGGCCACGCGCGCCACTCTCC |
| PstPc T141E FP | GACGACACGTTTGTCCAAGCGCTGGTCGACGAAGGCCG |
| PstPc T141E RP | CGGCCTTCGTCGACCAGCGCTTGGACAAACGTGTCGTC |
| pETDuet-PstP FP | CACC GCGGCCGCTCATATG GCGCGCGTGACCCTGG Not1 |
| pETDuet-PstPc RP | CGGTCACCAGTGCGGCCGCGAATGCTCACCGTCGGCC Not1 |
**Restriction sites/stop codon/mutated sequences have been underlined.