Fig. 4.
Effects of amino acid substitutions at D802 on channel gating properties. A, Voltage dependence of activation of BgNav1-1D802G and BgNav2-1G802D channels. B, Voltage dependence of activation of recombinant channels with substitutions at 802 other than D or G. The sodium currents were measured using a 20-ms test pulse ranging from −80 mV to+65 mV in 5-mV increments in BgNav1-1, BgNav2-1, and mutant channels. The values of half-maximal voltage for activation (V1/2) and slope factor (k) of BgNav1-1, BgNav2-1, and mutant channels are shown in Table 2. The normalized peak conductance was plotted against the potential of test pulses.