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. 2011 Feb;32(1):5–31.

Figure 12.

Figure 12.

Urine albumin measurement by LC-MS/MS. (A) Spectra and chromatogram of human and bovine serum albumin urine subjected to LC-MS. There is in-source breakage of intact albumin and the 3+ and 4+ charge species of the N-terminal 24 amino acid fragment give a high intensity, proteotypic signal in both cases. (B) and (C) Method comparison of LC-MS with immunoturbidimetry (B) and HPLC-UV (C). Reprinted from Babic et al. (Clin Chem 2006;52:2155–7) with permission from Clinical Chemistry.