Table 3.
A. Relative percent viability compared to the DMSO control | ||||
---|---|---|---|---|
Relative Extract Concentrationa | BRC-301 DC2.4 | BRC-304 DC2.4 | BRC-306 DC2.4 | BRC-301 bmDCs |
Low | 100 ±2 | 92 ±10 | 98 ±1 | 107 ±3 |
Medium | 99 ±2 | 85 ±2* | 94 ±1 | 116 ±5* |
High | 101 ±3 | 96 ±2 | 100 ±1 | 107 ±8 |
B. Relative percent viability compared to the LPS/DMSO control | ||||
Relative Extract Concentrationa | BRC-301 DC2.4 | BRC-304 DC2.4 | BRC-306 DC2.4 | BRC-301 bmDCs |
Low | 94 ±4 | 100 ±4 | 99 ±4 | 91 ±4 |
Medium | 97 ±5 | 102 ±3 | 92 ±2 | 97 ±1 |
High | 101 ±7 | 95 ±3 | 95 ±5 | 92 ±5 |
DC2.4 and bmDCs were cultured (n=3) in 6-well plates (1×106 per well) with DMSO, LPS (+DMSO), and/or varying concentrations of extracts for 48 hours. Viability of extract-treated cells was calculated as percent of either the DMSO-treated (A) or LPS/DMSO-treated (B) controls plus SEM. Data are representative of three experiments (n=4-6).
Extract concentrations varied among extracts as follows: for all extracts tested, the low and medium concentrations tested were 0.5 and 5.0 μg/ml, respectively. For BRC-301 and BRC-306, the high concentration tested was 25 μg/ml while 10 μg/ml was the highest concentration tested for BRC-304 due to decreased viability at higher concentrations.
indicates p < .05