Panels A and B: Left ventricular mid- myocardium immunohistochemically stained for the endothelial cell protein CD-34 (brown color). Algorithm thresholds were appropriately adjusted to allow microvascular evaluation within selected regions of interest. Panel A: 20x magnification, Panel B: 60X magnification
Panel C. Automatic completion of stained microvessels by the analysis algorithm enables measurements like vessel perimeter, lumen area etc.