Supp. Table S2.

Oligonucleotide primers used for PCR amplification of the novel missense mutations and polymorphisms

Location		Forward (5′>3′)#	Reverse (5′>3′)#	Annealing Temperatur e (°C)	PCR product size (bp)	Restriction enzyme
Mutation§
Ex. 9	c.953C>G	TTGGGTGATCCTTTTATTGTCA	CAACCCGCATCTCCCATGA*	58	195	Mnl I
Ex. 9	c.967G>A	AGTTGCCTTATGGGAGCTGC*	CACTGGCAAATCTTGCTTAAAA	60	229	Pst I
Ex. 10	c.1151T>C	GTTTGGATGAATCAGACTCAAAT	ACAAAAGTTTACCATGGTTCCA*	56	246	Bsr I
Ex. 13	c.1468T>A	TTCTGTCCACATGAGATGAGC	CCAACATTGAAATCCACCTTAT*	59	215	Bstx I
Polymorphism§
UTR 5′	c.42GC	GCTTCCTGGCAACGCCGATC*	ACTCATGGCCCTCTTCCTTT	62	309	Taq I
Ex. 1	c.61GC	GTCAGCATCAGCGGCCTCCT	ACTCATGGCCCTCTTCCTTT	62	415	Sma I
Ex. 1	c.75CA	GTCAGCATCAGCGGCCTCCT	ACTCATGGCCCTCTTCCTTT	62	415	Hga I
Intr. 1	c.195+34GT	GTCAGCATCAGCGGCCTCCT	ACTCATGGCCCTCTTCCTTT	62	415	Bbv I
Intr. 7	c.752+56TC	ATCTTGGTCATAAATTCAACAGC	GAGAATGTAATCAAATGGGGAGA	60	305	Taq I

Legend: Ex=exon, Intr=intron;

^§GALC gene GenBank-EM BL accession no. NM_000153.2 and no. NC_000014.8

^*primer modified so asto introduce a new restriction enzyme cleavage site according to Schwartz et al. (1991)