Fig. 4.
Micrographic pictures showing the intranuclear localization of phospho-Chk1S317 in HeLa cells; control (a–a′), a negative control section incubated with secondary antibodies only (b–b′), cells incubated for 24 h with 2.5 mM hydroxyurea (c–c′), cells incubated for 24 h with 10 mM hydroxyurea (d–d′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with 5 mM caffeine (e–e′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with the mixture of 2.5 mM hydroxyurea and 5 mM caffeine (f–f′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with 10 mM 2-aminopurine (g–g′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with the mixture of 2.5 mM hydroxyurea and 10 mM 2-aminopurine (h–h′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with 200 nM staurosporine (i–i′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with the mixture of 2.5 mM hydroxyurea and 200 nM staurosporine (j–j′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with 200 μM sodium metavanadate (k–k′), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated with the mixture of 2.5 mM hydroxyurea and 200 μM sodium metavanadate (l–l′). Scale bar = 10 μm