a Immunolabeling indices (%) estimated for HeLa cells stained with anti-Chk1S317 antibodies. Columns, mean from five independent experiments; bars, SD. * P = 0.009, compared with control (Mann–Whitney tests); # P = 0.009, compared with 2.5 mM HU (Mann–Whitney tests); & P = 0.03, compared with 2.5 mM HU (Mann–Whitney tests). b Quantification of anti-Chk1S317 pixel intensity per cell. Columns, mean from at least 108 cells per sample taken from five independent experiments; bars, SD. * P < 0.01, compared with control (Mann–Whitney tests); + P < 0.001, compared with control (Mann–Whitney tests); # P < 0.01 compared with 2.5 mM HU (Mann–Whitney tests); & P = 0.000215, compared with 2.5 mM HU (Mann–Whitney tests); $ P = 0.000038 compared with 2.5 mM HU (Mann–Whitney tests)