Fig. 9.

A Organization of the cellular microtubule network in HeLa cells; control (a), cells incubated for 24 h with 2.5 mM hydroxyurea or with 10 mM hydroxyurea (b), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 5 mM caffeine or with the mixture of 2.5 mM hydroxyurea and 5 mM caffeine (c), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 10 mM 2-aminopurine or with the mixture of 2.5 mM hydroxyurea and 10 mM 2-aminopurine (d), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 200 nM staurosporine or with the mixture of 2.5 mM hydroxyurea and 200 nM staurosporine (e), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 200 μM sodium metavanadate or with the mixture of 2.5 mM hydroxyurea and 200 μM sodium metavanadate (f). Scale bar = 10 μm. B Actin rearrangements occurred in HeLa cells during hydroxyurea-induced arrest and following PCC induction; control (a), cells incubated for 24 h with 2.5 mM hydroxyurea or with 10 mM hydroxyurea (b), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 5 mM caffeine or with the mixture of 2.5 mM hydroxyurea and 5 mM caffeine (c), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 10 mM 2-aminopurine or with the mixture of 2.5 mM hydroxyurea and 10 mM 2-aminopurine (d), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 200 nM staurosporine or with the mixture of 2.5 mM hydroxyurea and 200 nM staurosporine (e), cells incubated for 24 h with 2.5 mM hydroxyurea and post-treated either with 200 μM sodium metavanadate or with the mixture of 2.5 mM hydroxyurea and 200 μM sodium metavanadate (f). Scale bar = 10 μm