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. 2010 Sep;91(Pt 9):2167–2175. doi: 10.1099/vir.0.022640-0

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Fig. 2. — Detection of protein immunoprecipitated from FLAG–44-infected cell lysate by Western blotting. Lysate (Lys.) from AD169rv-infected (lane 1) and FLAG–44-infected (lane 2) cells and protein immunoprecipitated (IP) using an anti-FLAG antibody from those lysates (lanes 3 and 4, respectively) were separated on a 10 % polyacrylamide gel. Proteins in each lane were examined by Western blotting for the presence of UL44, FLAG, IRS1, TRS1 and β-actin using antibodies recognizing these proteins, as indicated to the right of the figure. The positions of IRS1 and TRS1 are indicated by arrows. The positions of molecular mass markers (in kDa) are indicated to the left of the figure.