Fig. 1.

Introduction of novel restriction enzyme sites into each segment of rgIA/30 SIV to differentiate it from Tx/98. The introduced novel restriction enzyme sites in each gene segment of the rgIA/30 SIV were used to differentiate it from the Tx/98 virus as shown in Table 1. RT-PCR was performed for RNA isolated from rgIA/30 and Tx/98, and the PCR products were incubated with different restriction enzymes as indicated and separated on agarose gels.