Fig. 2.

JJ3297-dependent restoration of IFN-β mRNA levels and inhibition of virus replication in MDCK cells. (a) Upper panel: cells were mock infected, treated with poly(I : C) or infected with influenza strain A/PR/8 at an m.o.i. of 2 and treated with increasing concentrations of JJ3297 as indicated or with 1 % DMSO (0 μM). After 6 h, cells were harvested for RT-PCR analysis of IFN-β and β-actin mRNA levels. Lower panel: cells were mock infected, treated with poly(I : C) or infected with influenza A/PR/8 at an m.o.i. of 2 and treated with either 1 % DMSO or 5 μM JJ3297 as indicated. After 6 h, cells were harvested for RT-PCR analysis of IFN-β and β-actin mRNAs. (b) Triplicate cultures were infected with A/PR/8 at an m.o.i. of 0.1 and treated with the indicated concentrations of JJ3297 or 1 % DMSO (0 μM). After 48 h, the supernatants were collected and analysed to determine TCID₅₀. (c) Triplicate cultures were incubated in the presence of JJ3297 at the indicated concentrations for 48 h. Cells were disrupted with CellTiter Glo reagent according to manufacturer's protocol and the luminescent signal was measured, indicating the extent of cell viability.