Fig. 6.

JJ3297 activity depends on a functional IFN response pathway. (a, b) Parental wild-type (WT) (a) or RNase L^−/− (b) MEFs were infected with A/PR/8-MA at an m.o.i. of 0.1 and treated with 5 μM JJ3297 or 1 % DMSO. After 48 h, the cells were fixed and stained for NP. DAPI was used to visualize cell nuclei. (c) Quantification of replication in WT or RNase L^−/− MEFs. Supernatants from cells infected with A/PR/8-MA at a low m.o.i. and treated with 5 μM JJ3297 or 1 % DMSO were harvested at 24 and 48 h time points and the virus titre was assayed for samples from three independent cultures by TCID₅₀ determination.