Fig. 2.
PCR-based detection of transfer of SV40 ori and SV40T sequences. Lane M, 100 bp DNA ladder (New England Biolabs); lanes 1 and 6, negative controls using ddH2O; lanes 2 and 7, templates of 50 ng DNA from transfected 293T producer cells as positive controls; lanes 3 and 8, 1 μl supernatant collected from 293T producer cells used as template to detect SV40 sequences; lanes 4 and 9, templates of 50 ng DNA extracted from transduced CEM-SS cells to detect the transfer of SV40 sequences into target cells.