Fig. 3.

Sensitivity to small-molecule CCR5 inhibitors of T/F HIV-1 Envs in pseudotyped-virus infection. Stocks of pseudotyped reporter viruses were prepared by co-transfecting 293T cells with Env expression constructs and plasmid pHIVΔEnv for 48 h and then harvested. TZM-bl cells were pre-treated with serially diluted CCR5 inhibitor maraviroc (a), CMPD-167 (b) or SCH-412147 (c) for 30 min before pseudotyped viruses were added. The luciferase activity of cell lysates was determined approximately 24 h later. The infectivity in medium alone was arbitrarily set to 100 % for each Env. Data are representative of three independent experiments, with each determination performed in triplicate (mean±sd).