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Microwave Settings and Times for the Coupling and Deprotection Steps
| step | power (W) |
temperature (°C) |
reaction time (s) |
|
|---|---|---|---|---|
| single couple | (1) | 35 | 75 | 300 |
| double couple | (1) | 35 | 75 | 300 |
| (2) | 35 | 75 | 300 | |
| Cys and His couple | (1) | 0 | 50 | 120 |
| 15 | 50 | 240 | ||
| Arg couple | (1) | 0 | 75 | 1,500 |
| 15 | 75 | 300 | ||
| (2) | 35 | 75 | 300 | |
| deprotection | (1) | 40 | 75 | 30 |
| (2) | 40 | 75 | 180 |
