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. Author manuscript; available in PMC: 2011 Mar 10.
Published in final edited form as: Nat Cell Biol. 2010 Aug 22;12(9):894–901. doi: 10.1038/ncb2093

Figure 2. KNL-1 is required to orient chromosomes on the acentrosomal meiotic spindle prior to anaphase onset.

Figure 2

(a) GFP-Aurora BAIR-2 and mCherry-histone-H2b dynamics in control embryos. Time is in seconds relative to anaphase I onset. Scale bar, 5 μm. (b) Top: Schematics of Aurora BAIR-2 signal on an individual chromosome and measurement of chromosome orientation angle using this signal; the spindle axis is defined by anaphase chromosome separation. Bottom: Stills from movies of the indicated conditions; quantification of individual chromosome angles relative to the axis of the meiosis I spindle, measured 20 sec before anaphase onset (n=12 embryos per condition), is presented on the right. Scale bars, 5 μm.