Table 1.
Mean levels of WBC DNA methylation in young girls using the MethyLight and LINE1 pyrosequencing assays by participant characteristics
| MethyLight | Pyrosequencing | ||||
| LINE1 | Sat2 | Alu | LINE1 | ||
| Breast cancer family history | |||||
| Yes (n = 31) | Mean, (SD) | 93.7, (16.9) | 125.7, (40.6) | 151.4, (35.9)* | 75.0, (4.4)* |
| No (n = 20) | Mean, (SD) | 98.4, (21.2) | 127.4, (31.6) | 169.8, (25.1) | 78.0, (3.5) |
| Breast Tanner stage | |||||
| 1–3 (n = 22) | Mean, (SD) | 99.4, (21.1) | 128.2, (37.5) | 155.5, (28.9) | 76.6, (4.2) |
| 4–5 (n = 28) | Mean, (SD) | 93.3, (16.3) | 125.7, (37.6) | 160.9, (36.9) | 75.7, (4.5) |
| Menarche | |||||
| No (n = 20) | Mean, (SD) | 97.0, (21.7) | 120.0, (36.5) | 155.1, (29.8) | 77.1, (3.4) |
| Yes (n = 31) | Mean, (SD) | 94.7, (16.7) | 130.5, (37.2) | 160.8, (35.4) | 75.6, (4.7) |
| Age at menarche (years) | |||||
| <12 (n = 16) | Mean, (SD) | 99.7, (16.1) | 128.9, (23.5) | 153.4, (23.4) | 77.2, (3.8) |
| ≥12 (n = 15) | Mean, (SD) | 89.3, (16.1) | 132.2, (48.7) | 168.7, (44.3) | 73.9, (5.1) |
| BMI (kg/m2) | |||||
| <23.8 (n = 31) | Mean, (SD) | 95.5, (21.4) | 130.4, (43.4) | 159.3, (36.6) | 75.6, (4.5) |
| ≥23.8 (n = 20) | Mean, (SD) | 95.6, (13.9) | 120.2, (23.6) | 157.5, (27.6) | 77.0, (3.9) |
*
p < 0.05.