Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Hepatol. 2010 Oct 29;54(3):506–512. doi: 10.1016/j.jhep.2010.07.020

Figure 3. Negative effect on Wnt pathway after Peg-IFN treatment is via upregulation of RanBP3.

Figure 3

A. TOPFlash luciferase assay showing a decrease in reporter activity after overexpression of RanBP3 in HepG2 cells.

B. Topflash luciferase reporter assay shows a significant decrease in β-catenin activity after Peg-IFN treatment in control siRNA transfected Hep3B cells (p<0.01). Transfection of RanBPB3 siRNA that leads to a dramatic suppression of RanBP3 protein expression after 48 hours (upper panel) led to a significant abrogation of Peg-IFN-induced reduction of TOPFlash reporter activity (p<0.05).