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. Author manuscript; available in PMC: 2012 Jan 30.
Published in final edited form as: Clin Chim Acta. 2010 Oct 27;412(3-4):277–285. doi: 10.1016/j.cca.2010.10.018

Table 2.

Suspected modification sites found on various samples of in vitro glycated HSA

Digest Residues Relevant Amino Acid Suspected Modification Sitesa
HSA-2 Trypsin 1–10 N+, K4, R10 N+ (FL-1H2O) + K4 (AFGP)
Trypsin 275–286 K276, K281, K286 K276 & K281 (FL & FL-1H2O)
Trypsin 539–557 or 542–560 K541, K545, K557
K545, K557, K560
K545 & K557 (FL-2H2O & FL-2H2O) or (FL-1H2O & Pyr)
K545 & K557 (FL-2H2O & Pyr) or (FL + CEL)
Glu-C 278–292 K281, K286 K281 & K286 (FL & CEL) or (FL-2H2O & Pyr)
Glu-C 83–100 K93, R98 K93 (FL-2H2O) or K93 & R98 (CEL & MG-H1)
Glu-C 566–585 or 168–188 K573, K574
K174, K181, R186
K573 and K574 (AFGP*2)
K174 & R186 (FL & ArgP)
Lys-C 196–205 K199, K205, R197 K199 & R197 (FL & 3-DG-H1/THP)
Lys-C 414–432 K414, K432, R428 K414 & R428 (FL-1H2O & AFGP) or K414 & R428 (AFGP and 3-DG-H1/THP)

HSA-5 Trypsin 520–525 K524, K525, R251 R521 & K524 (Pyr & G-H1)
Trypsin 275–286 K276, K281, K286 K276 & K281 (FL & FL-1H2O)
Glu-C 281–294 K281, K286 K281 & K286 (FL-2H2O & CEL)
Glu-C 426–442 K432, K436, K439, R428 R428 & K439 (G-H1 & Pyr) and (ArgP & CEL)
Glu-C 189–208 K190, K195, K199, K205, R197 K199 & R197 (Pyr & IB)
Lys-C 5–20 K12, K20, R10 K12 (FL)
Lys-C 414–432 K414, K432, R428 K414 & R428 (FL-1H2O & AFGP) or (AFGP & 3-DG-H1/THP)
Lys-C 525–534 K525, K534 K525 (FL-1H2O)

HSA-4 Trypsin 1–12 N+, K4, K12, R10 N+ & K4 (CEL & FL-2H2O) or N+ & R10 (FL-1H2O & MG-H1)
Trypsin or 200–212
or 403–413
K205, K212, R209
K413, R410
K205 & R209 (CML & ArgP)
K413 or R410 (AFGP)
Trypsin 198–209
or 182–195
or 206–218
K199, K205, R209
K190, K195, R186
K212, R209, R218
K199 & K205 AFGP, (FL & Pyr), or (FL-1H2O+FL-2H2O)
R186 & K190 (ArgP & Pyr)
R209 & K218 (FL-1H2O & MG-H1)
Trypsin 258–274 K262, K274 K262 (FL-1H2O)
Glu-C 312–321 K313, K317 K313 & K317 (AFGP & AFGP)
Glu-C 557–571 K557, K560, K564 K560 & K564 (FL & CML)
Glu-C 426–442 K432, K436, K439, R428 K439 (FL) or R428 & K439 (Pyr+MG-H1)
Glu-C 83–100 K93, R98 K93 (FL-2H2O) or K93 (CEL) & R98 (MG-H1)
Glu-C 189–208 K190, K195, K199, K205, R197 K199 & R197 (Pyr & IB)
a

The most likely modification sites, which were identified based on computational and structural data, are given in bold. The abbreviations for the types of glycation are defined in Table 1.