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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Dev Biol. 2010 Oct 28;349(2):440–450. doi: 10.1016/j.ydbio.2010.10.031

Figure 4. GusΔSOCS-Vasa interacts with discrete segments of Vasa protein.

Figure 4

(A) A graphic representation of each Vasa construct containing an N-terminal Metal Affinity Tag (MAT), C-terminal Flag epitope, corresponding domains and DEAD-box sequence motifs. (B) Gustavus binding domains in Vasa. Bacterial extracts containing N-term, DEAD and C-term recombinant Vasa proteins were incubated with immobilized GST, GST-GusΔSOCS wt or GST-GusΔSOCS R146W and bound proteins were analyzed by immunoblotting with anti-Flag antibodies. (C) A bipartite Gustavus-Vasa binding model in S. purpuratus. The gustavus B30.2/SPRY domain is shown in blue with binding surface A and binding surface B indicated as predicted previously (Woo et al., 2006a). A schematic representation of the S. purpuratus vasa DEAD box domain in orange, C-terminus domain in pink, N-terminus CCHC zinc knuckles in purple and unstructured glycine-rich flexible sequence in yellow.